Using the SynergyTMHT to Measure Time-Resolved Fluorescent Compounds

Introduction Under many experimental conditions autofluorescence of the sample or the microplate can cause unwanted background fluorescence. High background results in variable inter and intraexperimental signals, as well as a decrease in sensitivity. While several options are available to reduce background fluorescence, including the use of low-background microplates and different excitation and emission wavelengths, these measures often fail to adequately reduce the background signal. Another approach to this problem is to use time-resolved fluorescent reagents.